BIO 241 Genetics Laboratory Syllabus Spring 2002   Lab Projects: Laboratory technique -week 1 basic sterile technique, cell plating and streaking, dilutions, replica-platting Yeast genetics -weeks 1,3 testing genetic markers genetic crosses in fungi (diploid selection, tetrad dissection, gene mapping) Yeast Mutagenesis -weeks 2-8 Identification of genes responsible for maintenance of mitochondrial DNA, chemical mutagenesis of yeast cultures, genetic screen for temperature sensitive mutants based on carbon source metabolism and color assay, determine whether mutation is of nuclear or mitochondrial origin Restriction enzyme mapping -weeks 4-7 DNA restriction enzyme analysis agarose gel electrophoresis mapping sites of unknown section of DNA DNA fingerprinting -weeks 6-9 isolation of cheek cell DNA amplification of variable regions by PCR Molecular Genetics/Bioinformatics -weeks 7-9 detection of mtDNA by fluorescent microscopy (DAPI staining), DNA hybridization (Southern Blotting), radiochemical probing (32P) for mtDNA sequences analysis of unknown DNA sequence comparison with previously identified genes, Grading: Lab Oral Exam (based on experimental theory and procedure) 50 % Reports and Worksheets 50 % Return to Biology 240/241 Web page Return to Carleton College Home Page Createdby Joanne Penko and Nersi Nikakhtar Maintained by Sarah Deel (sdeel@carleton.edu) Last updated: